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wide field nikon eclipse ti2 microscope  (Nikon)


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    Structured Review

    Nikon wide field nikon eclipse ti2 microscope
    Wide Field Nikon Eclipse Ti2 Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 11824 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/wide+field+nikon+eclipse+ti2+microscope/ECLIPSE+Ti2/bio_rxiv__2025__11__06__685569-290-11-12
    Average 99 stars, based on 11824 article reviews
    wide field nikon eclipse ti2 microscope - by Bioz Stars, 2026-09
    99/100 stars

    Images

    Related Articles

    Imaging:

    Article Title: Genetically Encoded Red Fluorescent Indicators for Imaging Intracellular and Extracellular Potassium Ions
    Article Snippet: .. Imaging was performed at 20°C-25°C using an inverted wide-field Nikon Eclipse Ti2 microscope. ..

    Article Title: Genetically Encoded Red Fluorescent Indicators for Imaging Intracellular and Extracellular Potassium Ions
    Article Snippet: .. Imaging was conducted at20°C-25°C using an inverted wide-field Nikon Eclipse Ti2 microscope. ..

    Article Title: Genetically Encoded Red Fluorescent Indicators for Imaging Intracellular and Extracellular Potassium Ions
    Article Snippet: .. Imaging was conducted at 20°C-25°C using an inverted wide- field Nikon Eclipse Ti2 microscope. ..

    Article Title: Genetically Encoded Red Fluorescent Indicators for Imaging Intracellular and Extracellular Potassium Ions
    Article Snippet: .. Imaging was conducted at 20°C-25°C using an inverted wide-field Nikon Eclipse Ti2 microscope, capturing dynamic changes in both potassium and calcium signals over time. ..

    Article Title: Sensitive red fluorescent indicators for real-time visualization of potassium ion dynamics in vivo
    Article Snippet: .. Imaging was conducted 24–36 h posttransfection, utilizing an inverted wide-field Nikon Eclipse Ti2 microscope, equipped with a SPECTRA III light engine (Lumencor, USA) and an Orca Flash4.0v3 camera (Hamamatsu, USA), and operated via NIS-Elements AR software, utilizing 20× NA0.75 or 10× NA0.45 objective lens. .. In the process of intracellular validation, the transfected cells were initially incubated in a basal buffer solution composed of 1.5 mM CaCl 2 , 1.5 mM MgSO 4 , 1.25 mM NaH 2 PO 4 , 26 mM NaHCO 3 , and 10 mM d -Glucose (pH 7.4) with 15 μM valinomycin (Aladdin Biochemical Technology Co., China) and 5 μM CCCP (Shanghai Macklin Biochemical Co., China) for 15 min at 22–25 °C.

    Article Title: Genetically Encoded Red Fluorescent Indicators for Imaging Intracellular and Extracellular Potassium Ions
    Article Snippet: .. Imaging was conducted 24-36 h post-transfection, utilizing an inverted wide-field Nikon Eclipse Ti2 microscope, equipped with a SPECTRA III light engine (Lumencor, USA) and an Orca Flash4.0v3 camera (Hamamatsu, USA), and operated via NIS-Elements AR software, utilizing 20x NA0.75 or 10x NA0.45 objective lens. .. In the process of intracellular validation, the transfected cells were initially incubated in a basal buffer solution composed of 1.5 mM CaCl2, 1.5 mM MgSO4, 1.25 mM NaH2PO4, 26 mM NaHCO3 and 10 mM D-Glucose (pH 7.4) with 15 μM valinomycin (Aladdin Biochemical Technology Co., Ltd., China) and 5 μM CCCP (Shanghai Macklin Biochemical Co., Ltd., China) for 15 minutes at 22-25°C.

    Microscopy:

    Article Title: Genetically Encoded Red Fluorescent Indicators for Imaging Intracellular and Extracellular Potassium Ions
    Article Snippet: .. Imaging was performed at 20°C-25°C using an inverted wide-field Nikon Eclipse Ti2 microscope. ..

    Article Title: Genetically Encoded Red Fluorescent Indicators for Imaging Intracellular and Extracellular Potassium Ions
    Article Snippet: .. Imaging was conducted at20°C-25°C using an inverted wide-field Nikon Eclipse Ti2 microscope. ..

    Article Title: Genetically Encoded Red Fluorescent Indicators for Imaging Intracellular and Extracellular Potassium Ions
    Article Snippet: .. Imaging was conducted at 20°C-25°C using an inverted wide- field Nikon Eclipse Ti2 microscope. ..

    Article Title: Genetically Encoded Red Fluorescent Indicators for Imaging Intracellular and Extracellular Potassium Ions
    Article Snippet: .. Imaging was conducted at 20°C-25°C using an inverted wide-field Nikon Eclipse Ti2 microscope, capturing dynamic changes in both potassium and calcium signals over time. ..

    Article Title: Sensitive red fluorescent indicators for real-time visualization of potassium ion dynamics in vivo
    Article Snippet: .. Imaging was conducted 24–36 h posttransfection, utilizing an inverted wide-field Nikon Eclipse Ti2 microscope, equipped with a SPECTRA III light engine (Lumencor, USA) and an Orca Flash4.0v3 camera (Hamamatsu, USA), and operated via NIS-Elements AR software, utilizing 20× NA0.75 or 10× NA0.45 objective lens. .. In the process of intracellular validation, the transfected cells were initially incubated in a basal buffer solution composed of 1.5 mM CaCl 2 , 1.5 mM MgSO 4 , 1.25 mM NaH 2 PO 4 , 26 mM NaHCO 3 , and 10 mM d -Glucose (pH 7.4) with 15 μM valinomycin (Aladdin Biochemical Technology Co., China) and 5 μM CCCP (Shanghai Macklin Biochemical Co., China) for 15 min at 22–25 °C.

    Article Title: A structural and regulatory framework for Atg9-containing vesicle formation and their Atg1-dependent remodelling during autophagy initiation
    Article Snippet: Cells were diluted to an OD 600 of 0.05 and added to P96-1.5H-N plates (Cellvis) prior to imaging. .. Images were acquired in a temperature-controlled environment (30°C) on an inverted wide-field Nikon Eclipse Ti2 microscope, equipped with a 100X/1.4 NA lens and a Prime 95B sCMOS camera (Teledyne Photometrics), controlled through Micro-Manager 2.0 software . ..

    Article Title: Genetically Encoded Red Fluorescent Indicators for Imaging Intracellular and Extracellular Potassium Ions
    Article Snippet: .. Imaging was conducted 24-36 h post-transfection, utilizing an inverted wide-field Nikon Eclipse Ti2 microscope, equipped with a SPECTRA III light engine (Lumencor, USA) and an Orca Flash4.0v3 camera (Hamamatsu, USA), and operated via NIS-Elements AR software, utilizing 20x NA0.75 or 10x NA0.45 objective lens. .. In the process of intracellular validation, the transfected cells were initially incubated in a basal buffer solution composed of 1.5 mM CaCl2, 1.5 mM MgSO4, 1.25 mM NaH2PO4, 26 mM NaHCO3 and 10 mM D-Glucose (pH 7.4) with 15 μM valinomycin (Aladdin Biochemical Technology Co., Ltd., China) and 5 μM CCCP (Shanghai Macklin Biochemical Co., Ltd., China) for 15 minutes at 22-25°C.

    Software:

    Article Title: Sensitive red fluorescent indicators for real-time visualization of potassium ion dynamics in vivo
    Article Snippet: .. Imaging was conducted 24–36 h posttransfection, utilizing an inverted wide-field Nikon Eclipse Ti2 microscope, equipped with a SPECTRA III light engine (Lumencor, USA) and an Orca Flash4.0v3 camera (Hamamatsu, USA), and operated via NIS-Elements AR software, utilizing 20× NA0.75 or 10× NA0.45 objective lens. .. In the process of intracellular validation, the transfected cells were initially incubated in a basal buffer solution composed of 1.5 mM CaCl 2 , 1.5 mM MgSO 4 , 1.25 mM NaH 2 PO 4 , 26 mM NaHCO 3 , and 10 mM d -Glucose (pH 7.4) with 15 μM valinomycin (Aladdin Biochemical Technology Co., China) and 5 μM CCCP (Shanghai Macklin Biochemical Co., China) for 15 min at 22–25 °C.

    Article Title: A structural and regulatory framework for Atg9-containing vesicle formation and their Atg1-dependent remodelling during autophagy initiation
    Article Snippet: Cells were diluted to an OD 600 of 0.05 and added to P96-1.5H-N plates (Cellvis) prior to imaging. .. Images were acquired in a temperature-controlled environment (30°C) on an inverted wide-field Nikon Eclipse Ti2 microscope, equipped with a 100X/1.4 NA lens and a Prime 95B sCMOS camera (Teledyne Photometrics), controlled through Micro-Manager 2.0 software . ..

    Article Title: Genetically Encoded Red Fluorescent Indicators for Imaging Intracellular and Extracellular Potassium Ions
    Article Snippet: .. Imaging was conducted 24-36 h post-transfection, utilizing an inverted wide-field Nikon Eclipse Ti2 microscope, equipped with a SPECTRA III light engine (Lumencor, USA) and an Orca Flash4.0v3 camera (Hamamatsu, USA), and operated via NIS-Elements AR software, utilizing 20x NA0.75 or 10x NA0.45 objective lens. .. In the process of intracellular validation, the transfected cells were initially incubated in a basal buffer solution composed of 1.5 mM CaCl2, 1.5 mM MgSO4, 1.25 mM NaH2PO4, 26 mM NaHCO3 and 10 mM D-Glucose (pH 7.4) with 15 μM valinomycin (Aladdin Biochemical Technology Co., Ltd., China) and 5 μM CCCP (Shanghai Macklin Biochemical Co., Ltd., China) for 15 minutes at 22-25°C.



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    Image Search Results


    key resources table

    Journal: Cell reports

    Article Title: Non-cell-autonomous regulation of mTORC2 by Hedgehog signaling maintains lipid homeostasis

    doi: 10.1016/j.celrep.2024.115191

    Figure Lengend Snippet: key resources table

    Article Snippet: Imaging of GRD-3::mKate2, LGG-1::GFP, HLH-30::GFP, DAF-16::mKate2, and GFP::PQM-1 was performed on a Nikon Ti2 wide-field microscope equipped with a Hamamatsu ORCA-Fusion BT camera.

    Techniques: Virus, Recombinant, SYBR Green Assay, Transfection, DC Protein Assay, Mutagenesis, CRISPR, Plasmid Preparation, Software